Neuronal Culture

• Maintenance and coordination of the use of facilities and common equipment for neural cultures.
• Detect, analyze and resolve user needs
• Monitoring, advice and updating of the regulations established in the installations of neural cultures and type 2 biosecurity activities
• Technical support in the use of equipment and specific procedures for nervous system cultures
• Mandatory training for self-service users (see conditions and rates)
• Carrying out experimental procedures intended for subsequent qualitative and quantitative evaluation through the evaluation of different cellular parameters
• Advice and support in placing orders for specialized material and reagents
• Advice and technical support in the design of experiments, protocols, the analysis of the data obtained and the interpretation of the results of the experimental procedures
• Support in INc's scientific dissemination activities
• Study and evaluation of new techniques and scientific advances in the field of neuroscience for the INc technical laboratory platform

• Primary cell culture
• Cell lines                                      
• Available cell collection
• Other techniques related to cell cultures

Currently, the platform has three full-equipped cell culture rooms, one specialized for working with cell lines, another dedicated for primary cultures, and a third adaptable room versatile for both procedures. In addition, these laboratories have general equipment available for all platform users, located in accessory rooms needed to properly develop the cell culture work:

• M2/103. Cell lines room
• M2/104. Mixed room
• M2/130.2. Primary cell culture room
• General equipment in all room
• Associated equipment to cell culture rooms

Featured equipment

• Amaxa nucleofector ® II
• GenePulser XCell
• FLoid
• Scepter 3.0 TM
• Cytometer FC-500
• Cell Injury Controller II (CIC II)

The facilities have been accredited by the Ministry to work with GMOs within the biosafety level 2

When requesting trainings, staff-operated experiments, or use of facilities, the INc-UAB members will have priority over non-members. Upon request, members or non-members who have not previously used common laboratories or equipment will have preference over those who have recently done so.

Another important criterion will be the order of reception of requests and the possibility of the technician to intersperse them with other requests. If is not possible to intercalate orders, the technical staff will give priority to INc-UAB members and those who have not previously used the laboratories.

Reservations of the equipment of the platform can be made by two different ways: by e-mail (inc.cultius@uab.cat), or by the intranet once the potential users have passed the mandatory training (Please consult with the technical staff to register for the latter).

The culture laboratories are regularly open from 9 a.m. to 5 p.m., although users who work under self-operated conditions and are authorized by the staff can use it outside this established schedule, on weekends and holidays (as long as they have correctly issued the appropriate authorization to have access during weekends and holidays).

The costs of training or conducting experimental procedures are divided into 2 items:

• Material used: fungible (reagents and other basic material) not supplied by the researcher, animals, if applicable and the use of equipment.
• Technician working hours: different fees will apply depending on the link that the user has with the UAB and the type of project in which fees will be charged

Cell culture rates

General rates