Bacterium capable of aquifer decontamination identified in River Besòs

UAB researchers have succeeded in characterising and cultivating, for the first time in Europe, a bacterium of the genus Dehalogenimonas, which transforms certain toxic organochlorine compounds into others that are harmless, thus opening the door to their production and application to contaminated aquifers.

15/10/2015

This bacterial genus was first described not long ago – in 2009 – and only two strains had previously been isolated, in chloroalkane-contaminated aquifers in Louisiana, USA. This is the first description of the characterisation and culturing of Dehalogenimonas in Europe, though sequences of its genome have been identified in various locations, such as the Arctic Ocean, the Baltic Sea, Canada, China, Germany, Hungary, Spain, Taiwan and the USA.

These bacteria can only use organochlorine compounds as an energy source during their respiration process, transforming them into products that are less chlorinated, more biodegradable and, in some cases, harmless.

From River Besòs sediments, after three years’ research, the researchers have obtained a stable bacterial culture and have shown its capacity to transform some of the chlorinated aliphatic hydrocarbons that are frequently found in aquifers, such as 1,2-dichloroethane and 1,2-dichloropropane, into harmless products like ethene and propene, respectively.

The researchers also used an analytic technique to determine the fractionation of the stable isotopes of one of these contaminants, thus demonstrating in situ that the bacteria contribute to the disappearance of the contaminants, distinguishing this process from naturally occurring physicochemical ones involving dilution of the contaminants by rainwater, adsorption to the organic matter or volatilisation.

Obtaining the bacterial consortium opens the way to production and subsequent application in contaminated aquifers, using the strategy of bioaugmentation, which involves adding bacteria with specific catabolic capacities in order to eliminate the contaminants. Although this technology has never been used in Catalonia, it is regarded as mature, with hundreds of successes in the USA and Canada.

“Being able to cultivate these bacteria in Europe means significantly lowering the costs of applying them, as most of the companies that deal in them are based in North America. Furthermore, if you take into account that they grow best in the anoxic conditions of aquifers, and that bioaugmentation is a low-cost, efficient technique, compatible with other remediation techniques, these bacteria could even be eventually applied at source, in the industrial plants themselves", explains Ernest Marco, the research coordinator.

A serious environmental problem
Contamination of aquifers by organochlorine compounds is one of the most serious environmental problems in this country and the rest of Europe. These contaminants are widely used in industry and reach subterranean waters by accident or as a result of improper waste disposal. Once in the aquifers, they can build up for years because of their low biodegradability, posing a threat due to their high toxicity. According to data from the Waste Agency of Catalonia, 8% of contaminated soils recorded in 2014 contained organochlorine compounds, of which 77 surpassed the maximum benchmark concentrations.

This study led by Dr Ernest Marco-Urrea and published in Environmental Science & Technology, was conducted at the Universitat Autònoma de Barcelona (Department of Chemical Engineering and Department of Genetics and Microbiology) in collaboration with the University of Barcelona (Research Group in Applied Mineralogy and Fluid Geochemistry) and the Helmholtz Centre for Environmental Research – UFZ (ISOBIO Department) in Leipzig, Germany).

Reference: Martín-González et al (2015). Stable Carbon Isotope Fractionation During 1,2-Dichloropropane-to-Propene Transformation by an Enrichment Culture Containing Dehalogenimonas Strains and a dcpA Gene. Environ Sci Technol. 21; 49(14):8666-74. doi: 10.1021/acs.est.5b00929.